G Rex Bioreactor

The invention is directed to producing large numbers of cells using hollow fiber bioreactor technology The cells are nonembryonic stem, nongerm cells that can be characterized by one or more of.

G rex bioreactor. Singleuse Bioreactor Systems Speed and flexibility in cell culture operations from process development to largescale biomanufacturing Increased facility utilization Singleuse equipment reduces the need for costly and timeconsuming cleaning and validation procedures between batches. The GRex cell culture platform is based on a gaspermeable membrane technology that provides numerous advantages over other systems Conventional bioreactor platform technologies developed for large scale mammalian cell expansion are typically constrained by the mechanics of delivering oxygen to an expanding cell population. Gas Permeable Rapid Expansion (GRex;.

End User – Biopharmaceutical Companies, Biotechnology Companies, Other End Users;. Largescale cRBC production in culture dishes is practically impossible;. GRex®6M Well Plate – $ Each well can expand 5 million cells into between 0 and 400 million cells in about 10 days without medium exchange Cytokine addition may be needed every 34 days, depending upon your cell type Individual Well Specifications 10cm 2 gas permeable membrane surface area with 110 ml max fill volume.

It is obvious from Table 4 that the expansion of TIL even in bigger numbers is possible, up to more than 10 10 TIL might be grown in flasks as well as in bags, Grex flasks, Wave bioreactors, or ZRP perfusion bioreactors Media and supplements are qualitatively similar In all examples, use of feeder cells seem to be essential. On the other hand, GRex gaspermeable membranebased bioreactors provide a more physiologic environment and avoid the risk and cost associated with more complex systems. The use of GRex M devices in combination with our newly optimized protocol allows the operator to initiate a suspension culture with a small number of cells (125 × 10 5 cells/cm 2 ) and achieve a 100fold expansion in only 10 days using just 10 ml of culture medium per cm 2 significantly exceeding that previously achieved in tissue culture plates or bags 13,,21,25 Furthermore, since cells were supplemented with medium as a onetime upfront addition, we were able to utilize glucose.

Largescale cRBC production in culture dishes is practically impossible;. Therefore, a GRex bioreactor from Wilson Wolf Manufacturing (Saint Paul, MN) was used in which a gaspermeable membrane at the bottom allowed proliferation in larger volume/surface conditions 29, 30 The GRex bioreactor does not support adherent cells, such as the CD14 PBMC that promote erythroid yield by increasing CD34 cell survival, 18 which compromises stage 1 yield (data not shown). Microparticles in combination with the GRex® bioreactor system and varying dose schedules of IL2 Additionally, cells were Additionally, cells were cultured in serumfree media conditions after determining the best seeding density, and cytokine dosing schedule.

With support from NHLBIPACT, we have now defined optimized cell culture parameters that can be used in the GRex M series, which reliably produces 100fold cell expansion in only 10 days without media replenishment. Bioreactors of the Z ® RP system can be operated in the GMP Breeder The platform automatically regulates key parameters of cultivation processes (pH, pO 2, medium temperature, medium perfusion, feeding rate) Thus, the perfusion bioreactor process guarantees homogeneous supply of cultured cells with nutrients and gasses. Summary T cells can be transduced with retroviral vectors in the GRex bioreactor Clinically relevant levels of transgene expression can be achieved by combining reagents in the GRex, without complicated coating steps or timeconsuming spinning steps.

GRex® is the only production platform created specifically to produce immune cells such as T cells, natural killer cells, and hematopoietic cells GRex® is proving to be the most practical and cost effective way to scale up and scale out We welcome your input as we work to make GRex® the gold standard for immune cell production. Cells Type Mammalian Cells, Bacterial Cells, Yeast Cells, Other Cells;. A cell expansion system for culturing and expanding cells in hydrogel tubes is disclosed herein The cell expansion system allows for expanding cells that can significantly reduce the production time and cost, while increase the production capacity.

Molecule Type Monoclonal Antibodies (MABS), Vaccines, Stem Cells, GeneModified. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators. GRex®24 Well Plate P/N M $9868 each (sold individually) Individual well specifications 2cm² gas permeable membrane with 8mL media capacity Expand 1 million cells into between 40 to 80 million cells in about 12 days with only two exchanges of medium GAMMA IRRADIATED For nonclinical, nontherapeutic, research use only.

The GRex cell culture platform is based on a gaspermeable membrane technology that provides numerous advantages over other systems Conventional bioreactor platform technologies developed for. New generation GRex10 bioreactors and describe optimal culture conditions This large scale myeloid DC generation could significantly contribute to the clinical applicability of DCs in cancer. Microparticles in combination with the GRex® bioreactor system and varying dose schedules of IL2 Additionally, cells were Additionally, cells were cultured in serumfree media conditions after determining the best seeding density, and cytokine dosing schedule.

Expansion of CART cells using GRex bioreactors GRex is a r elatively new platform It is a cell culture ask with a gaspermeable. Largescale cRBC production in culture dishes is practically impossible;. Advances in technologies have seen platforms such as the following being evaluated in cell therapy bioprocessing applications gaspermeable vessels (eg GRex (Wilson Wolf, New Brighton, MN, USA)) , rocking motion bioreactors (eg Xuri Cell Expansion System (GE Healthcare, Chicago, IL, USA)), and contained, integrated bioprocess platforms (eg CliniMACS Prodigy (Miltenyi Biotec, Bergisch Gladbach, Germany)) Further to this, technologies used for the concentration of Tcell populations.

Expansion of CART cells using GRex bioreactors GRex is a relatively new platform It is a cell culture flask with a gaspermeable membrane at the base that allows cells to grow to a high density without compromising gas exchange. In this study, we investigate the compatibility and efficiency of ex vivo T cell expansion from human peripheral blood mononuclear cells (PBMCs) using the novel Cloudz ™ CD3/CD28 dissolvable polymer microparticles in combination with the GRex ® bioreactor system and varying dose schedules of IL2 Additionally, cells were cultured in serum. Therefore, a GRex bioreactor from Wilson Wolf Manufacturing (Saint Paul, MN) was used in which a gaspermeable membrane at the bottom allowed proliferation in larger volume/surface conditions 29, 30 The GRex bioreactor does not support adherent cells, such as the CD14 PBMC that promote erythroid yield by increasing CD34 cell survival, 18 which compromises stage 1 yield (data not shown).

Wilson Wolf’s revolutionary products include unique geometry that allows medium to reside at heights not previously possible with conventional methods And, unlike any other device, GRex® geometry provides cells with virtually unlimited access to oxygen and nutrients on demand. On the other hand, GRex gaspermeable membranebased bioreactors provide a more physiologic environment and avoid the risk and cost associated with more complex systems. Wilson Wolf Corporation, Saint Paul, MN, USA) bioreactors are disposable singleuse vessels specifically engineered to culture and expand immune cells Like gaspermeable bags, GRex vessels are dependent on incubation in standard cell culture incubators.

Wilson Wolf Corporation, Saint Paul, MN, USA) bioreactors are disposable singleuse vessels specifically engineered to culture and expand immune cells Like gaspermeable bags, GRex vessels are dependent on incubation in standard cell culture incubators. The GRex cell culture platform is based on a gaspermeable membrane technology that provides numerous advantages over other systems Conventional bioreactor platform technologies developed for. The bioreactor is at maximum cell density, an angle of 7° or 8° might be needed when using Cellbag2L, 10L, or L For Cellbag50L, an angle of 6° to 7° can be used when at maximum density Increase the rocking angle and decrease speed if excessive foaming is observed Experience shows.

Using the elegant GRex culture platform, cellular therapies can be scaled up to bring immunotherapy from the benchtop to the bedside and treat large patient. GE Healthcare Life Sciences), which utilizes a rocking platform, has been used to expand the CD19targeted CAR T cell therapy CTL,,30 Another culture system that can be used is the GRex (Wilson Wolf), which has the ability to expand cells from low seeding densities31,32 The GRex uses gaspermeable. Using the elegant GRex culture platform, cellular therapies can be scaled up to bring immunotherapy from the benchtop to the bedside and treat large patient.

Gas Permeable Rapid Expansion (GRex;. CAR Tcell therapy uses autologous T cells expanded to therapeutic volumes (liter scale) in rocking bed, disposable bag bioreactors such as WAVE™ or CultiBag bioreactors Technologies for the automated or semiautomated processing of CAR T cells are commercially available and include the CliniMACS Prodigy, DynaMag, and GRex systems 27. The WAVE Bioreactor (now known as the Xuri;.

PBMCs from 5 healthy donors were used as starting material for running the simplified GRexbased process in parallel with the standard bag based process to generate 10 total lots of cell product An MOI of 5 was used for all of the largescale transductions Transduction in the GRex entails mixing reagents and adding the solution to cells. Summary T cells can be transduced with retroviral vectors in the GRex bioreactor Clinically relevant levels of transgene expression can be achieved by combining reagents in the GRex, without complicated coating steps or timeconsuming spinning steps. Advances in technologies have seen platforms such as the following being evaluated in cell therapy bioprocessing applications gaspermeable vessels (eg GRex (Wilson Wolf, New Brighton, MN, USA)) , rocking motion bioreactors (eg Xuri Cell Expansion System (GE Healthcare, Chicago, IL, USA)), and contained, integrated bioprocess platforms (eg CliniMACS Prodigy (Miltenyi Biotec, Bergisch Gladbach, Germany)) Further to this, technologies used for the concentration of Tcell populations.

(d) Generation of multiviral antigenspecific T cells using GRex bioreactor Donor PBMCs are pulsed with 15mer peptides mix spanning EBV, adenovirus, CMV, BK virus and human herpes virus antigen. GRex GRex100MCS 750mL medium addition via gravity fill Duration A singleuse bioreactor with novel design and features to accommodate modern cell culture processes Duration 607. Wilson Wolf Corporation, Saint Paul, MN, USA) bioreactors are disposable singleuse vessels specifically engineered to culture and expand immune cells Like gaspermeable bags, GRex vessels are dependent on incubation in standard cell culture incubators.

Recently, the G‐Rex membrane system has been used to expand large numbers of T‐cells, NK cells, HSPCs, and other cell lines within a scalable closed system 28 33 The gas‐permeable membrane provides efficient oxygen transfer from the incubator atmosphere to the cells. Gas Permeable Rapid Expansion (GRex;. Wilson Wolf is dedicated to helping advance the field of healthcare through use of our revolutionary GRex gas permeable cell culture device platform.

CAR Tcell therapy uses autologous T cells expanded to therapeutic volumes (liter scale) in rocking bed, disposable bag bioreactors such as WAVE™ or CultiBag bioreactors Technologies for the automated or semiautomated processing of CAR T cells are commercially available and include the CliniMACS Prodigy, DynaMag, and GRex systems 27. The propensity among clinical programs has been to employ easy‐to‐use platforms such as T‐flasks, G‐rex bioreactors, and static culture bags However, it is widely recognized that such systems have poor monitoring and control capability and are limited with respect to scalability. Therefore, a GRex bioreactor from Wilson Wolf Manufacturing (Saint Paul, MN) was used in which a gaspermeable membrane at the bottom allowed proliferation in larger volume/surface conditions 29,30 The GRex bioreactor does not support adherent cells, such as the CD14 PBMC that promote erythroid yield by increasing CD34 cell survival, 18 which compromises stage 1 yield (data not shown).

PBMCs from 5 healthy donors were used as starting material for running the simplified GRexbased process in parallel with the standard bag based process to generate 10 total lots of cell product An MOI of 5 was used for all of the largescale transductions Transduction in the GRex entails mixing reagents and adding the solution to cells. Gas Permeable Rapid Expansion (GRex;. Here we demonstrate a novel method for transduction of activated T cells with Vectofusin1 reagent Transduction is accomplished in suspension, in GRex bioreactors The simplified transduction step is integrated into a streamlined process that uses a single bioreactor with limited operator intervention.