G Rex Flask

Gas permeable growth area CellBIND® Surface treated 560 mL maximum working volume 17 cm 2 available growth area Corning HYPERFlask is a High Yield Performance Flask with a gas permeable cell growth surface area of 17 cm 2 The rectangular vessel has the external.

G rex flask. Using the GRex culture flask supplied by Wilson Wolf, we can now readily grow sufficient quantities of these cells for therapeutic purposes The GatheRex pump which we are requesting is the only system compatible with the Grex culture flasks and allows for the concentration and harvesting of the Tcells at the end of the culture. GRex flasks is of advantage for TIL expansion by providing high O 2 concentration near to the sedimented TIL that enhances growth. A TIL rapid expansion protocol (REP) was developed using 500 mL capacity flasks with a 100 cm² gaspermeable silicone bottom, GRex100 TIL growth was successfully initiated in GRex10 flasks from tumor digests from 13 of 14 patients and from tumor fragments in all 11 tumor samples tested.

To overcome this limitation, Wilson Wolf Manufacturing developed the GRex, a cell culture flask with a gaspermeable membrane at the base that supports large media volumes without compromising gas exchange. GRex devices allow cells to obtain oxygen and nutrients on demand while retaining the simplicity of traditional flasks The net result is a practical cellproduction process with a tremendous. GRex® multiwell technology is ideal for applications involving nonadherent cells Compared to traditional multiwell plates, advantages of the GRex® multiwell plates include nutrient availability that is uncoupled from oxygen delivery leading up to 100fold cell expansion in less time with fewer manipulations.

If cells are in a flask, the flask can be agitated and rapped against the heel of your hand to dislodge the cells If using culture dishes or wells, pipet the PBS forcefully against the culture surface to dislodge the cells Collect the cells in PBS and pool with the previously collected cells. For GRexbased cultures, 30–60 tumor fragments were seeded per GRex 100M flask (Wilson Wolf Manufacturing Corporation) When preREP was initiated using cryopreserved tissue in 10% dimethyl sulfoxide (DMSO) cryopreservation solution, the tissue was thawed at 37°C in complete media supplemented with Pulmozyme. Recently, major efforts have been deployed to resolve this issue The use of the Gaspermeable flask (GRex) during the REP has been one application that has facilitated this process.

GRex® multiwell technology is ideal for applications involving nonadherent cells Compared to traditional multiwell plates, advantages of the GRex® multiwell plates include nutrient availability that is uncoupled from oxygen delivery leading up to 100fold cell expansion in less time with fewer manipulations. GRex culture device (a) shows the limited gas exchange that occurs in conventional cultureware, which limits the volume of media and consequently the available nutrients In contrast the GRex provides gas exchange from the base of the flask which allows cells to be cultured with a superior ratio of media per surface area. With the purpose of overcoming this limitation, Wilson Wolf Manufacturing has developed a Gaspermeable Rapid Expansion device (GRex), which is essentially a flask with a gaspermeable silicone membrane at the base that allows efficient O 2 and CO 2 exchange.

5×10 6 TIL were seeded per GREX flask on day 0 and the expansion was completed using same reagents, feeding schedule and cell ratios as the current protocol performed in traditional flask Rapid expansion of TIL by aAPC. To overcome this limitation, Wilson Wolf Manufacturing developed the GRex, a cell culture flask with a gaspermeable membrane at the base that supports large media volumes without compromising. To overcome this limitation, Wilson Wolf Manufacturing developed the GRex, a cell culture flask with a gaspermeable membrane at the base that supports large media volumes without compromising.

And, unlike any other device, GRex® geometry provides cells with virtually unlimited access to oxygen and nutrients on demand See the Revolution in Action Advantages of Our Technology Providing virtually unlimited oxygen and nutrients on demand is particularly beneficial for the culture of nonadherent cells when compared to prior methods. In this review we discuss current protocols for the ex vivo expansion of virus and tumorspecific T cells and describe our experience in manufacture optimization using a gaspermeable static culture flask (GRex). To overcome this limitation, Wilson Wolf Manufacturing developed the GRex, a cell culture flask with a gaspermeable membrane at the base that supports large media volumes without compromising.

A TIL rapid expansion protocol (REP) was developed using 500 mL capacity flasks with a 100 cm² gaspermeable silicone bottom, GRex100 TIL growth was successfully initiated in GRex10 flasks from tumor digests from 13 of 14 patients and from tumor fragments in all 11 tumor samples tested. To overcome this limitation, Wilson Wolf Manufacturing developed the GRex, a cell culture flask with a gaspermeable membrane at the base that supports large media volumes without compromising gas exchange. The WAVE Bioreactor (now known as the Xuri;.

On day 9, triVSTs were harvested and restimulated in a GRex100 gas permeable flask Cryopreservation was performed after the second stimulation on days 16–18 of culture (B) Transgene expression after the 1st and 2nd stimulation was determined by flow cytometry in 4 donors. Flask $2159 $2699 Stainless steel and a sleek sixounce size means these cool and compact G Rex Stainless Steel Flasks travel along wherever you journey Take them to the neighbors backyard party or off to the big game, all you do is fill and go. EZ Flask for hybridomas Higher yields of monoclonal antibody Depending on the antibody, media and incubation time, yields can be up to 40 x higher compared Tflasks with no passaging required Simple, easy and fast Just fill and inoculate No need for cell passaging In 2 to 4 weeks the process is complete with minimal handson.

Flask, shown in Figure 1B In GRex, cells 10A reside upon a gas permeable membrane 15A A gas A gas membrane support A holds the membrane in a horizontal position so that cells distribute uniformly. Various culture vessels, including GRex flasks (Wilson Wolf Manufacturing), cell differentiation bags and bioreactors have proven suitability for Tcell manufacturing 5 Zhan H. We evaluated three different flask sizes with surface areas and maximum media volumes of 10cm2(40 mL or 4mL per cm2) for the GRex40, 100cm2(500 mL or 5mL per cm2) for the GRex 500, and 100cm2(00 mL or mL per cm2) GRex00, respectively (Figure 3c) Figure 3.

Various culture vessels, including GRex flasks (Wilson Wolf Manufacturing), cell differentiation bags and bioreactors have proven suitability for Tcell manufacturing,,. The use of the Gaspermeable flask (GRex) during the REP has been one application that has facilitated this process Here we show that the use of this new device is able to rescue poor TIL growth and maintain clonal diversity while supporting an improved mitochondrial function. Jin et al 24 proposed the method of primary culture and the rapid expansion of TIL by using the gaspermeable flask (GREX flask) to greatly promote the production of TIL (Fig 2) Significant curative effects can be obtained by combining IL2 with excited antibodies against 41BB and CD3 to culture TILs (Fig 2).

For TIL propagation carried in the GREX 100M flask (Wilson Wolf Manufacturing, New Brighton, MN);. Wilson Wolf is dedicated to helping advance the field of healthcare through use of our revolutionary GRex gas permeable cell culture device platform. On day 9, triVSTs were harvested and restimulated in a GRex100 gas permeable flask Cryopreservation was performed after the second stimulation on days 16–18 of culture (B) Transgene expression after the 1st and 2nd stimulation was determined by flow cytometry in 4 donors.

To overcome this limitation, Wilson Wolf Manufacturing developed the GRex, a cell culture flask with a gaspermeable membrane at the base that supports large media volumes without compromising gas. With the purpose of overcoming this limitation, Wilson Wolf Manufacturing has developed a Gaspermeable Rapid Expansion device (GRex), which is essentially a flask with a gaspermeable silicone membrane at the base that allows efficient O 2 and CO 2 exchange. GRex culture device (a) shows the limited gas exchange that occurs in conventional cultureware, which limits the volume of media and consequently the available nutrients In contrast the GRex provides gas exchange from the base of the flask which allows cells to be cultured with a superior ratio of media per surface area.

Recently, major efforts have been deployed to resolve this issue The use of the Gaspermeable flask (GRex) during the REP has been one application that has facilitated this process. With the purpose of overcoming this limitation, Wilson Wolf Manufacturing has developed a Gaspermeable Rapid Expansion device (GRex), which is essentially a flask with a gaspermeable silicone membrane at the base that allows efficient O 2 and CO 2 exchange. We evaluated three different flask sizes with surface areas and maximum media volumes of 10cm2(40 mL or 4mL per cm2) for the GRex40, 100cm2(500 mL or 5mL per cm2) for the GRex 500, and 100cm2(00 mL or mL per cm2) GRex00, respectively (Figure 3c) Figure 3.

GRex®6M Well Plate – P/N M $ each (sold individually) Individual well specifications 10cm² gas permeable membrane with a 100mL media capacity Expand 5 million cells into between 0 to 400 million cells in about 10 days with NO medium exchange GAMMA IRRADIATED For nonclinical, nontherapeutic, research use only. EZ Flask for hybridomas Higher yields of monoclonal antibody Depending on the antibody, media and incubation time, yields can be up to 40 x higher compared Tflasks with no passaging required Simple, easy and fast Just fill and inoculate No need for cell passaging In 2 to 4 weeks the process is complete with minimal handson. GRex® is the only production platform created specifically to produce immune cells such as T cells, natural killer cells, and hematopoietic cells GRex® is proving to be the most practical and cost effective way to scale up and scale out We welcome your input as we work to make GRex® the gold standard for immune cell production.

To overcome this limitation, Wilson Wolf Manufacturing developed the GRex, a cell culture flask with a gaspermeable membrane at the base that supports large media volumes without compromising gas. Bioreactors offer several advantages the GRex flask is a closedsystem cylindrical vessel with optimized gas exchange and sampling options and permits rapid expansion of cells in an incubator,. If cells are in a flask, the flask can be agitated and rapped against the heel of your hand to dislodge the cells If using culture dishes or wells, pipet the PBS forcefully against the culture surface to dislodge the cells Collect the cells in PBS and pool with the previously collected cells.

Human monocytederived DC (mDC) loaded with peptides, protein, tumor cell lysates, or tumor cell RNA, are being tested as vaccines against multiple human malignancies and viral infection with great promise One of the factors that has limited more widespread use of these vaccines is the need to generate mDC in large scale Current methods for the largescale cultivation of mDC in static. The GRex flasks are GMPcompliant and are scalable up to 1L flasks which are qualified as an FDA Class 1 medical device allowing full closed process manufacture. To overcome this limitation, Wilson Wolf Manufacturing developed the GRex, a cell culture flask with a gaspermeable membrane at the base that supports large media volumes without compromising gas exchange.